Web10. The following day, dilute 1 mM stock solution MitoTracker® Red CMXRos directly into growth media at a final concentration 200 nM and incubate for 30 min at 37 °C. 11. Remove media from wells and wash twice with PBS. Notes: Place needed amounts of 100% methanol on ice prior at the beginning of this day. Web19 dec. 2024 · To label the mitochondria or lysosome, the HK-2 cells were cultured on cell slides in a 24-well plate. The HK-2 cells were treated with MitoTracker Red (500 nM), MitoTracker Green (50 nM), or LysoTracker Red (50 nM) and Hoechst (5 μg/mL), following the manufacturer’s instructions. The cells were fixed and immunostained with the antibody.

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WebLysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. Rat LAMP1 fused to enhanced green fluorescent protein (EGFP) mostly accumulate … Web13 apr. 2024 · As shown in Figure 3A, the red fluorescence was from CBT-SiPc, and the green fluorescence from the commercial LysoTracker Green, MitoTracker, and lipid … chelmsford tourism

LysoTracker and MitoTracker Red a... preview & related info

WebNational Center for Biotechnology Information WebLysoTracker Red DND-99 is a cell-permeable red fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Red DND-99 has an excitation and emission maximum of 577/590 nm and can be … WebLysoTracker and MitoTracker Red are fluorescent probes widely used for viable cell staining of lysosomes and mitochondria, respectively. They are utilized to study organelle localization and their resident proteins, assess organelle functionality and quantification of organelle numbers. chelmsford tourist attractions